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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Smith A C, Gilman R L

Comp Biochem Physiol B. 1982;71(3):337-43. doi: 10.1016/0305-0491(82)90392-3.

Nuclear lens tissue from fishes has been a valuable source of polymorphic proteins. 2. Generally, only the water-soluble proteins have been utilized. The present study describes (a) a solution that extracts both the water-soluble and water-insoluble proteins, and (b) an electrophoretic analysis of these proteins from 13 fish species to test for polymorphism. 3. The electrophoretic method includes among its features membranes that (a) consist of a gel form of cellulose acetate, and (b) are treated with glycine to reduce protein adsorption. 4. Electrophoretic patterns revealed distinct species differences. Polymorphism related to size of fish (a little documented subject) was not found in three of the species, but was demonstrated in varying degrees by the other ten species. 5. The methodology of this study is applicable to diverse biological disciplines, viz, population biology, taxonomy, and developmental biology.

鱼类的晶状体核组织一直是多态性蛋白质的宝贵来源。2. 一般来说，仅利用了水溶性蛋白质。本研究描述了（a）一种能提取水溶性和水不溶性蛋白质的溶液，以及（b）对来自13种鱼类的这些蛋白质进行电泳分析以检测多态性。3. 该电泳方法的特点包括（a）由醋酸纤维素凝胶形式组成的膜，以及（b）用甘氨酸处理以减少蛋白质吸附。4. 电泳图谱显示出明显的物种差异。在其中三个物种中未发现与鱼的大小相关的多态性（一个记录较少的主题），但其他十个物种在不同程度上表现出多态性。5. 本研究的方法适用于多种生物学学科，即群体生物学、分类学和发育生物学。

Smith A C, Gilman R L

Comp Biochem Physiol B. 1982;71(3):337-43. doi: 10.1016/0305-0491(82)90392-3.

Nuclear lens tissue from fishes has been a valuable source of polymorphic proteins. 2. Generally, only the water-soluble proteins have been utilized. The present study describes (a) a solution that extracts both the water-soluble and water-insoluble proteins, and (b) an electrophoretic analysis of these proteins from 13 fish species to test for polymorphism. 3. The electrophoretic method includes among its features membranes that (a) consist of a gel form of cellulose acetate, and (b) are treated with glycine to reduce protein adsorption. 4. Electrophoretic patterns revealed distinct species differences. Polymorphism related to size of fish (a little documented subject) was not found in three of the species, but was demonstrated in varying degrees by the other ten species. 5. The methodology of this study is applicable to diverse biological disciplines, viz, population biology, taxonomy, and developmental biology.

鱼类的晶状体核组织一直是多态性蛋白质的宝贵来源。2. 一般来说，仅利用了水溶性蛋白质。本研究描述了（a）一种能提取水溶性和水不溶性蛋白质的溶液，以及（b）对来自13种鱼类的这些蛋白质进行电泳分析以检测多态性。3. 该电泳方法的特点包括（a）由醋酸纤维素凝胶形式组成的膜，以及（b）用甘氨酸处理以减少蛋白质吸附。4. 电泳图谱显示出明显的物种差异。在其中三个物种中未发现与鱼的大小相关的多态性（一个记录较少的主题），但其他十个物种在不同程度上表现出多态性。5. 本研究的方法适用于多种生物学学科，即群体生物学、分类学和发育生物学。