Amako K, Umeda A, Murata K
J Bacteriol. 1982 May;150(2):844-50. doi: 10.1128/jb.150.2.844-850.1982.
The arrangement of peptidoglycan in the cell wall of Staphylococcus was observed with the newly developed freeze-fracture technique, using n-octanol instead of water as the freezing medium. The replica of the trichloroacetic acid-extracted cell wall (TCA-wall) showed two areas. One of them has a concentric circular structure, a characteristic surface structure of the staphylococcal cell wall, and the other showed an irregular and rough surface. The chemical analysis of the wall revealed that the TCA-wall consisted of mostly peptidoglycan. By digesting the TCA-wall with lysozyme, the circular structures were greatly disturbed, and they disappeared after 60 min of treatment. From these observations it can be expected that the peptidoglycan is arranged in a concentric circular manner in the newly generated cell wall of Staphylococcus.
使用新开发的冷冻断裂技术，以正辛醇代替水作为冷冻介质，观察了葡萄球菌细胞壁中肽聚糖的排列。三氯乙酸提取的细胞壁（TCA壁）的复制品显示出两个区域。其中一个具有同心圆形结构，这是葡萄球菌细胞壁的特征性表面结构，另一个显示出不规则且粗糙的表面。对细胞壁的化学分析表明，TCA壁主要由肽聚糖组成。用溶菌酶消化TCA壁后，圆形结构受到极大干扰，处理60分钟后消失。从这些观察结果可以预期，肽聚糖在葡萄球菌新生成的细胞壁中呈同心圆形排列。
