Hase S, Okawa K, Ikenaka T
J Biochem. 1982 Feb;91(2):735-7. doi: 10.1093/oxfordjournals.jbchem.a133748.
Ovomucoid isolated from egg white of japanese quail contained mannose, galactose, glucosamine, and sialic acid (8.6 : 1.3 : 6.6 : 0.5 mol/mol) as sugar components. Sugar chains were liberated from the polypeptide portion by hydrazinolysis, N-acetylated and fractionated on Bio-Gel P-2. The reducing ends of the thus obtained sugar moieties were pyridylaminated. Chemical and exoglycosidase digestion studies of the fluorescent pyridylamino derivative of a major sugar fraction indicated the following structure for one of the major sugar chains of japanese quail ovomucoid; Man alpha 1 leads to 6(Man alpha 1 leads to 3)Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc.
从日本鹌鹑蛋清中分离出的卵类黏蛋白含有甘露糖、半乳糖、氨基葡萄糖和唾液酸（摩尔比为8.6 : 1.3 : 6.6 : 0.5）作为糖成分。通过肼解从多肽部分释放出糖链，进行N - 乙酰化并在Bio - Gel P - 2上分级分离。将由此获得的糖部分的还原端进行吡啶基胺化。对一种主要糖馏分的荧光吡啶基氨基衍生物进行化学和外切糖苷酶消化研究，得出日本鹌鹑卵类黏蛋白一种主要糖链的以下结构：Manα1→6(Manα1→3)Manβ1→4GlcNAcβ1→4GlcNAc。
