Bäck O, Larsen A
J Invest Dermatol. 1982 Apr;78(4):309-12. doi: 10.1111/1523-1747.ep12507383.
Contact sensitivity to picryl chloride was investigated by means of the ear swelling test and a radiometric test in order to establish optimal experimental conditions for these assays. Contact sensitivity was demonstrated as soon as 2 days after sensitization, with a maximum reaction 3-4 days after sensitization, when a 48 hr test reaction was registered. The test reaction was followed for 72 hr and maximum was arrived at after 24 hr and 48 hr for the ear swelling test and the radiometric test, respectively. Optimal sensitization was reached with a 7% solution of picryl chloride and a maximum test reaction was found with 0.75-1.0% picryl chloride. It is concluded that both assays measure contact sensitivity in quantitative terms and a future replacement of the guinea pig maximization test is discussed.
为了确定这些检测方法的最佳实验条件，通过耳部肿胀试验和放射性检测法对氯化苦基的接触敏感性进行了研究。致敏后2天即可显示接触敏感性，致敏后3 - 4天出现最大反应，此时记录48小时的试验反应。对试验反应进行了72小时的跟踪，耳部肿胀试验和放射性检测法分别在24小时和48小时后达到最大值。用7%的氯化苦基溶液可达到最佳致敏效果，用0.75 - 1.0%的氯化苦基溶液可发现最大试验反应。得出的结论是，这两种检测方法都能定量测量接触敏感性，并讨论了未来替代豚鼠最大化试验的问题。
