Miyachi Y, Takigawa M
J Invest Dermatol. 1982 May;78(5):363-5. doi: 10.1111/1523-1747.ep12507465.
The role of Langerhans cells in the induction of contact photosensitivity (CPS) to tetrachlorosalicylanilide (TCSA) was investigated in mice. CPS was induced by 2 daily paintings of 50 microliters of 1% TCSA in acetone plus black light irradiations for 2.5 hr. Both ears were challenged with 20 microliters of 0.1% TCSA in ethanol plus black light irradiation for 2.5 hr on day 5, and ear thickness was read at 24 hr. The density of LCs detected as ATPase-positive cells dramatically decreased 2 days after exposure to UVB. CPS was not induced by painting the photoallergen to the skin which had been pre-irradiated with UVB. The ear swelling response returned to the normal level when the mice were sensitized 12 days after UVB exposure in accordance with the complete regeneration of ATPase-positive cells. Dose of UVB in the present study did not affect the development of CPS through systemic mechanism. These demonstrations indicate that LCs play an important role in the induction of CPS.
在小鼠中研究了朗格汉斯细胞在对四氯水杨酰苯胺（TCSA）诱导接触性光过敏（CPS）中的作用。通过每天两次涂抹50微升1%的TCSA丙酮溶液并进行2.5小时的黑光照射来诱导CPS。在第5天，双耳用20微升0.1%的TCSA乙醇溶液进行激发并进行2.5小时的黑光照射，24小时后测量耳厚度。检测为ATP酶阳性细胞的朗格汉斯细胞密度在暴露于UVB后2天显著降低。将光变应原涂抹于预先用UVB照射过的皮肤上不能诱导CPS。当小鼠在UVB暴露12天后致敏时，耳部肿胀反应恢复到正常水平，这与ATP酶阳性细胞的完全再生一致。本研究中UVB的剂量未通过全身机制影响CPS的发生。这些结果表明朗格汉斯细胞在CPS的诱导中起重要作用。
