Norris D A, Capin L, Weston W L
J Invest Dermatol. 1982 May;78(5):386-90. doi: 10.1111/1523-1747.ep12507510.
The effect of epicutaneous methyl prednisolone (MP) at 10-4, 10-5, and 10-6 molar concentration was studied in 54 normal, healthy volunteers using a new, in vivo microchemotaxis technique. Significant inhibition of monocyte chemotaxis occurred at all concentrations studied and persisted over a 24-hr period with 10-4 molar MP. Neutrophil chemotaxis was significantly inhibited only with 10-4 MP. The inhibitory effect of MP on neutrophil and monocyte chemotaxis occurred earlier and at lower concentrations if the skin sites were pretreated with steroid. Thus, when corticosteroids are applied on abraded skin in concentrations achievable in vivo, monocyte chemotaxis into tissue is inhibited for longer periods and at lower drug concentrations than is neutrophil chemotaxis. By avoiding the significant systemic effects of corticosteroids on circulating monocyte and neutrophil populations, these experiments establish that local inhibition of chemotaxis is an important anti-inflammatory effect of corticosteroids, with differential effect on monocytes and neutrophils.
采用一种新的体内微趋化技术，对54名正常健康志愿者研究了10-4、10-5和10-6摩尔浓度的表皮甲基泼尼松龙（MP）的作用。在所研究的所有浓度下，单核细胞趋化均受到显著抑制，且10-4摩尔MP在24小时内持续存在这种抑制作用。仅10-4 MP显著抑制中性粒细胞趋化。如果皮肤部位用类固醇预处理，MP对中性粒细胞和单核细胞趋化的抑制作用出现得更早且浓度更低。因此，当以体内可达到的浓度将皮质类固醇应用于擦伤皮肤时，单核细胞向组织的趋化在比中性粒细胞趋化更长的时间内且在更低的药物浓度下受到抑制。通过避免皮质类固醇对循环单核细胞和中性粒细胞群体的显著全身作用，这些实验证实局部趋化抑制是皮质类固醇的一种重要抗炎作用，对单核细胞和中性粒细胞有不同的作用。
