Van Wyck D B, Witte M H, Witte C L
J Infect Dis. 1982 Apr;145(4):514-9. doi: 10.1093/infdis/145.4.514.
To examine the interplay between the spleen and complement activation in host defense against pneumococcemia, serial colony counts in blood and survival rates were studied after high-dose (10(5)) and low-dose (10(2)) intravenous challenge with growth-phase Streptococcus pneumoniae type 3 in rats with splenic remnants of varying size (partial, total, or no splenic resection) and with or without complement depletion (by pretreatment with cobra venom factor [CVF]). After high-dose challenge, the combination of total splenectomy and CVF produced greater pneumococcemia and higher mortality than either factor alone. After low-dose challenge, survival was uniform except when CVF was coupled with total splenectomy or splenic remnants less than one third of normal size, combinations which resulted in extremely high early mortality. This synergistic interaction between total splenectomy and CVF reveals a serious postsplenectomy immune defect and provides a highly sensitive assay of residual protective function in the spleen.
为研究脾脏与补体激活在宿主抵御肺炎球菌血症中的相互作用，在用不同大小脾脏残余（部分脾切除、全脾切除或未行脾切除）以及有或无补体耗竭（通过眼镜蛇毒因子[CVF]预处理）的大鼠中，经静脉给予高剂量（10⁵）和低剂量（10²）对数生长期3型肺炎链球菌进行攻击后，对血液中的系列菌落计数和生存率进行了研究。高剂量攻击后，全脾切除与CVF联合使用比单独使用任一因素导致更严重的肺炎球菌血症和更高的死亡率。低剂量攻击后，除了CVF与全脾切除或小于正常大小三分之一的脾脏残余联合使用时，生存率是一致的，这两种联合导致极高的早期死亡率。全脾切除与CVF之间的这种协同相互作用揭示了严重的脾切除后免疫缺陷，并提供了一种对脾脏残余保护功能高度敏感的检测方法。
