[Effect of phenobarbital and chlorpromazine on bile flow and bile acid excretion in male Wistar rats].

Bile flow and excretion of monohydroxy-, dihydroxy- and trihydroxy-bile acids (MBA, DBA and TBA) were estimated after acute and subacute phenobarbital and chlorpromazine pretreatment in 60-day-old male Wistar rats. Bile was collected in bile-fistula rats in three 1-hour periods. MBA were not detected. Neither single nor repeated ip. administration of different amounts of saline before bile sampling nor oral water supply within the bile collection period influenced the bile flow and excretion of DBA and TBA. Phenobarbital administration (60 mg/kg b.w. ip.) 2 hrs before bile sampling did not influence bile flow and bile acid (BA) excretion. After 3 days pretreatment with phenobarbital (3 X 60 mg/kg b.w. ip.) the bile flow was somewhat increased, BA-excretion was unchanged and the relation TBA/DBA diminished. Chlorpromazine administration (40 mg/kg b.w. ip.) 1,5 hrs before bile sampling decreased bile flow and BA excretion within the first collection period, whereas bile flow and BA excretion increased in the thrid collection period. No signs of cholestasis were observed after chlorpromazine pretreatment once a day for 3 days. Bile flow and BA excretion were increased and the relation TBA/DBA was unchanged.