Schulze H P, Wagner H
Acta Biol Med Ger. 1978;37(2):249-57.
The experiments were designed to determine the amount of free amino acids which passes through the placenta from the maternal pool in plasma to the fetus. On day 19 of gestation we determined the growth rate of the rat fetus to be about 39 microgram amino nitrogen/minute/litter. Uniformly labelled [14 C]-L-leucine was used in our experiments. The specific radioactivity was measured in the maternal plasma amino acids and in fetal amino acids and proteins from zero to sixty minutes after pulse labelling. By means of compartmental analysis we calculated the transfer rate of amino nitrogen from the mother to the fetus to be 89 microgram/min/litter. The rate of amino nitrogen acccumulation in fetal carcass proteins was 39 microgram amino nitrogen/min/litter. The importance of our results in fetal metabolism is discussed.
这些实验旨在确定从母体血浆库通过胎盘进入胎儿体内的游离氨基酸的量。在妊娠第19天，我们测定大鼠胎儿的生长速率约为39微克氨基氮/分钟/窝。我们的实验使用了均匀标记的[14C]-L-亮氨酸。在脉冲标记后0至60分钟内，测定母体血浆氨基酸以及胎儿氨基酸和蛋白质中的比放射性。通过房室分析，我们计算出氨基氮从母体向胎儿的转移速率为89微克/分钟/窝。胎儿胴体蛋白质中氨基氮的积累速率为39微克氨基氮/分钟/窝。讨论了我们的结果在胎儿代谢中的重要性。
