去甲肾上腺素对小牛心脏浦肯野纤维动作电位浓度相关效应的离子基础。
The ionic basis of concentration-related effects of noradrenaline on the action potential of calf cardiac purkinje fibres.
作者信息
Kass R S, Wiegers S E
出版信息
J Physiol. 1982 Jan;322:541-58. doi: 10.1113/jphysiol.1982.sp014054.
Abstract
- Noradrenaline has paradoxical concentration-dependent effects on the Purkinje fibre action potential. At concentrations greater than 500 nM, it shortens the action potential whereas at lower concentrations it prolongs the action potential. 2. We determined the dose--response relation for Isi to noradrenaline in Purkinje fibres and found that a noradrenaline concentration of 126 nM produces a half maximal effect. 3. A maintained component of Isi was shown to have a similar response to noradrenaline. 4. The dose-dependence of noradrenaline-induced changes in Ix was determined. The half maximal noradrenaline concentration for this relationship was shown to be 133 nM. 5. The dose--response relationships for Ix and Isi were not altered by variation in extracellular Ca2+. 6. Ix appears to be approximately twice as responsive as Isi to saturating doses of noradrenaline. 7. These data were incorporated in a computer-generated reconstruction of the effects of noradrenaline on the action potential and are sufficient to account for the paradoxical effects of noradrenaline.
摘要
- 去甲肾上腺素对浦肯野纤维动作电位具有矛盾的浓度依赖性效应。浓度大于500 nM时，它会缩短动作电位，而在较低浓度时则会延长动作电位。2. 我们测定了浦肯野纤维中内向整流电流（Isi）对去甲肾上腺素的剂量反应关系，发现126 nM的去甲肾上腺素浓度产生半数最大效应。3. 结果表明，Isi的持续成分对去甲肾上腺素具有类似的反应。4. 测定了去甲肾上腺素诱导的Ix变化的剂量依赖性。该关系的半数最大去甲肾上腺素浓度为133 nM。5. 细胞外Ca2+的变化未改变Ix和Isi的剂量反应关系。6. 对于饱和剂量的去甲肾上腺素，Ix的反应似乎约为Isi的两倍。7. 这些数据被纳入计算机生成的去甲肾上腺素对动作电位影响的重建中，足以解释去甲肾上腺素的矛盾效应。
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