Nagle G T
Life Sci. 1982 Mar 8;30(10):803-7. doi: 10.1016/0024-3205(82)90592-6.
Ganglia dissected from the marine bivalve, Macrocallista nimbosa, were pooled and incubated in a series of seawater media designed to promote or inhibit the release of the neuropeptide Phe-Met-Arg-Phe-NH2 (FMRFamide). The amount of peptide released, determined by radioimmunoassay (RIA), was enhanced two to seven times when the medium contained high potassium. FMRFamide released was reversibly inhibited when calcium was omitted from the high potassium medium. Immunoreactive FMRFamide was found in Macrocallista hemolymph in a concentration (10(-9)M) that excites isolated Macrocallista ventricles. Although the in vivo release sites have not yet been localized, these findings already show that the endogenous neuropeptide FMRFamide can be characterized as a neurohormone or as a neurotransmitter in Macrocallista.
从海洋双壳贝类“云美巨蛎（Macrocallista nimbosa）”中分离出的神经节被集中起来，并在一系列旨在促进或抑制神经肽苯丙氨酸 - 蛋氨酸 - 精氨酸 - 苯丙氨酸 - 酰胺（FMRFamide）释放的海水培养基中进行孵育。通过放射免疫测定法（RIA）测定，当培养基中含有高钾时，释放的肽量会增加两到七倍。当从高钾培养基中去除钙时，释放的FMRFamide会受到可逆抑制。在云美巨蛎的血淋巴中发现了具有免疫活性的FMRFamide，其浓度（10⁻⁹M）能刺激分离出的云美巨蛎心室。尽管体内释放位点尚未定位，但这些发现已经表明，内源性神经肽FMRFamide在云美巨蛎中可被表征为一种神经激素或神经递质。
