Too C K, Croll R P
Department of Physiology and Biophysics, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada.
Cell Tissue Res. 1995 Aug;281(2):295-304. doi: 10.1007/BF00583398.
FMRFamide-like immunoreactivity was detected histochemically in the sea scallop Placopecten magellanicus. Most immunoreactivity was concentrated in the cerebral, pedal, and parietovisceral ganglia, particularly in the cortical cell bodies and in their fibers which extend into the central neuropile. Whole-mount immunofluorescence studies were used to localize concentrations of immunoreactive cells on the dorsal and ventral surfaces of each ganglion. Immunoreactivity was also detected in nerves emanating from the ganglia. Strong immunoreactivity was localized in peripheral organs, including the gut and gills of juvenile and adult scallops. Weak immunoreactivity was detected in the gonads, heart, and adductor muscle of the adults. A broad FMRFamide-like immunoreactive band of 2.5-8.2 kDa was detected by Western blotting of acetone extracts of the parietovisceral ganglia. In the presence of protease inhibitors, two FMRFamide-like immunoreactive bands (7.2-8.2 kDa and > 17 kDa) were obtained. Neither of these bands comigrated with the FMRFamide standard. It is concluded that peptides of the FMRFamide family are probably regulators of numerous central and peripheral functions in P. magellanicus.
通过组织化学方法在大西洋扇贝（Placopecten magellanicus）中检测到了类FMRF酰胺免疫反应性。大多数免疫反应性集中在脑、足和内脏神经节，特别是在皮质细胞体及其延伸至中枢神经纤维网的纤维中。采用整装免疫荧光研究来定位每个神经节背侧和腹侧表面免疫反应性细胞的集中区域。在从神经节发出的神经中也检测到了免疫反应性。强免疫反应性定位于外周器官，包括幼体和成体扇贝的肠道和鳃。在成体的性腺、心脏和闭壳肌中检测到弱免疫反应性。通过对内脏神经节丙酮提取物进行蛋白质印迹分析，检测到一条2.5 - 8.2 kDa的宽类FMRF酰胺免疫反应性条带。在存在蛋白酶抑制剂的情况下，获得了两条类FMRF酰胺免疫反应性条带（7.2 - 8.2 kDa和> 17 kDa）。这些条带均未与FMRF酰胺标准品共迁移。得出的结论是，FMRF酰胺家族的肽可能是大西洋扇贝众多中枢和外周功能的调节因子。
