Zacks S I, Sheff M F
Muscle Nerve. 1982 Feb;5(2):152-61. doi: 10.1002/mus.880050213.
The effect of increasing age on the completeness of anterior tibial muscle regeneration from autotransplants of minced muscle has been studied in Swiss Webster and C57/B6J mice aged 18 to 120 days. A progressively declining capability to regenerate new myofibers was associated with a decreasing phagocytic clearance of implanted myofiber debris. Concurrently, there was decreased presumptive myoblast proliferation and new myofiber formation. The importance of age-related host factors, including nonspecific macrophage activity, in muscle mince regeneration was demonstrated by: (1) the successful regeneration of muscle in heterotransplanted muscle minces from older mice implanted in younger animals and (2) the failure of muscle regeneration when the reverse experiment was performed in syngeneic animals. Heterologous striated muscle from the diaphragm regenerated in the bed of the excised anterior tibial muscle, whereas heterologous cardiac muscle failed to regenerate as expected because of the absence of satellite cells. The failure of phagocytic clearance of implanted myofiber mince and concurrent retardation of regeneration suggests a major age-related nonimmune role of phagocytic macrophages in the early stages of regeneration of anterior tibial muscle from isotopic minced muscle implants.
在18至120日龄的瑞士韦伯斯特小鼠和C57/B6J小鼠中，研究了年龄增长对切碎肌肉自体移植后胫前肌再生完整性的影响。新肌纤维再生能力的逐渐下降与植入肌纤维碎片的吞噬清除减少有关。同时，假定的成肌细胞增殖和新肌纤维形成减少。年龄相关的宿主因素，包括非特异性巨噬细胞活性，在肌肉切碎物再生中的重要性通过以下方式得到证明：（1）将老年小鼠的异种移植肌肉切碎物植入年轻动物体内后肌肉成功再生；（2）在同基因动物中进行反向实验时肌肉再生失败。来自膈肌的异源横纹肌在切除的胫前肌床中再生，而异源心肌由于缺乏卫星细胞未能如预期那样再生。植入肌纤维切碎物的吞噬清除失败以及再生的同时延迟表明，吞噬性巨噬细胞在同基因切碎肌肉植入后胫前肌再生早期具有主要的与年龄相关的非免疫作用。
