Blomquist E, Arro E, Brunk U
Acta Pathol Microbiol Scand A. 1978 May;86(3):257-63. doi: 10.1111/j.1699-0463.1978.tb02041.x.
A human diploid glia line (U-787 CG) was cultured on haptotactic islands using a miniclone method recently described. The surface morphology and motility pattern of the cells were studied using timelapse cinemicrophotography and scanning electron microscopy. Proliferating cells showed locomotion and ruffling activity with intermittent associated macropinocytosis. Non-dividers, viz, cells which had not divided 10 days after seeding on the haptotactic islands, were immobile, larger than the dividers, rather flat with few microvilli, and showed only very occasional ruffles almost completely without associated macropinocytosis. Our present findings show that phase III cells growing singly on haptotactic islands in serum-containing medium behave like phase II cells starved of growth factors or density growth inhibited. The results corroborate the theory that stationary cells have depressed ruffling activity and associated macropinocytosis as compared with proliferating cells.
使用最近描述的微型克隆方法，在趋触性岛状结构上培养人二倍体神经胶质细胞系（U - 787 CG）。使用延时电影显微镜摄影术和扫描电子显微镜研究细胞的表面形态和运动模式。增殖细胞表现出运动和褶皱活动，并伴有间歇性相关的巨吞饮作用。非分裂细胞，即在趋触性岛状结构上接种10天后未分裂的细胞，不活动，比分裂细胞大，相当扁平，微绒毛少，并且几乎完全没有相关巨吞饮作用，仅偶尔出现褶皱。我们目前的研究结果表明，在含血清培养基中趋触性岛状结构上单独生长的III期细胞，其行为类似于缺乏生长因子或密度生长受抑制的II期细胞。结果证实了这样的理论，即与增殖细胞相比，静止细胞的褶皱活动和相关巨吞饮作用受到抑制。
