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The in vitro cultivation of Taenia pisiformis to sexually mature adults.

作者信息

Osuna-Carrillo A, Mascaró-Lazcano M C

出版信息

Z Parasitenkd. 1982;67(1):67-71. doi: 10.1007/BF00929515.

DOI:10.1007/BF00929515
PMID:7072322
Abstract

Taenia pisiformis was cultivated from a cysticercus to a non-fertile adult in a diphasic medium. This medium was composed of coagulated bovine serum, as a solid phase, and CMRL 1066, modified by the addition of fetal calf serum, yeast extract, glucose and KCl, as a liquid phase. The medium was gassed with 10% O2 and 5% CO2 in N2. Evidence of morphogenesis with proglottid formation occurred after 7-8 days of culture, and genital pores were formed after 22 days. Some worms showed signs of teratological changes with two or three genital pores.

摘要

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本文引用的文献

1
An in vitro technique for the production of eggs of Echinococcus granulosus by maturation of partly developed strobila.一种通过部分发育的节片成熟来生产细粒棘球绦虫虫卵的体外技术。
Parasitology. 1966 Nov;56(4):763-6. doi: 10.1017/s003118200007178x.
2
In vitro culture of the strobilar state of Echinococcus granulosus (sheep strain): a review of basic problems and results.细粒棘球绦虫(绵羊株)成虫节片状态的体外培养:基本问题与结果综述
Int J Parasitol. 1974 Dec;4(6):631-44. doi: 10.1016/0020-7519(74)90028-9.
3
Occurrence of physiological strains of Echinococcus granulosus demonstrated by in vitro culture of protoscoleces from sheep and horse hydatid cysts.
通过对来自绵羊和马包虫囊肿的原头节进行体外培养证明细粒棘球绦虫生理株的存在。
Int J Parasitol. 1974 Aug;4(4):443-5. doi: 10.1016/0020-7519(74)90057-5.
4
Studies on the in vitro culture of Taenia crassiceps.
Int J Parasitol. 1976 Apr;6(2):143-9. doi: 10.1016/0020-7519(76)90071-0.