Zwilling B S, Campolito L B, Reiches N A
Am Rev Respir Dis. 1982 Apr;125(4):448-52. doi: 10.1164/arrd.1982.125.4.448.
Mononuclear phagocytes are functionally heterogeneous. To study the relationship of the heterogeneous populations of macrophages from the lung, alveolar macrophages from Syrian hamsters that had been immunized and rechallenged intratracheally with Mycobacterium bovis (Strain BCG) were separated by discontinuous albumin gradient centrifugation into 5 subpopulations designated A, B, C, D, and E. An activated alveolar macrophage subpopulation (defined by the ability to destroy tumor target cells) was enriched only in fraction D. Cells in fraction D destroyed 40% of the tumor cells, whereas unseparated alveolar macrophages destroyed 29%. Fractions A, B, C, and E destroyed less than 15% of the tumor cells. The subpopulations were functionally heterogeneous with regard to chemotactic responsiveness, Fc receptor activity, and phagocytic activity. Incubation of the subpopulations with a lymphocyte supernatant from spleen cells stimulated with concanavalin A enhanced the cytotoxic activity of fraction D and activated cells in fractions C and E to destroy tumor cells. Neither resident alveolar macrophages nor any of the subpopulations destroyed tumor cells. Only resident fraction D cells killed tumor cells when incubated with lymphokine containing supernatant fluids. The results are consistent with the hypothesis that some subpopulations of alveolar macrophages may be related and exist as a continuum of differentiation.
单核吞噬细胞在功能上具有异质性。为了研究来自肺部的巨噬细胞异质群体之间的关系，将经牛分枝杆菌（卡介苗菌株）气管内免疫和再次攻击的叙利亚仓鼠的肺泡巨噬细胞，通过不连续白蛋白梯度离心法分离为5个亚群，分别命名为A、B、C、D和E。一个活化的肺泡巨噬细胞亚群（由破坏肿瘤靶细胞的能力定义）仅在D组分中富集。D组分中的细胞破坏了40%的肿瘤细胞，而未分离的肺泡巨噬细胞破坏了29%。A、B、C和E组分破坏的肿瘤细胞少于15%。这些亚群在趋化反应性、Fc受体活性和吞噬活性方面在功能上是异质的。将这些亚群与用伴刀豆球蛋白A刺激的脾细胞的淋巴细胞上清液一起孵育，增强了D组分的细胞毒性活性，并激活了C和E组分中的细胞以破坏肿瘤细胞。驻留肺泡巨噬细胞和任何亚群都不破坏肿瘤细胞。只有驻留的D组分细胞在与含有淋巴因子的上清液孵育时杀死肿瘤细胞。这些结果与以下假设一致，即肺泡巨噬细胞的一些亚群可能相关，并作为一个连续的分化过程存在。
