Iwata M, Sasaki H, Kishino Y, Tsuboi T, Sugishita T, Hosokawa T
Anal Quant Cytol. 1982 Mar;4(1):72-6.
Using human uterine cervical carcinoma cells transplanted in nude mice and mice leukemia L5178Y cells, changes in the cell cycle following irradiation were observed by flow cytometry (FCM), and changes in the cell nuclei during the course of irradiation were measured by FCM. Experiments in vivo as well as in vitro caused accumulation of cells in the G2 to M populations, resulting in the so-called G2 block phenomenon as revealed by FCM analysis of DNA distributions. The radiation-induced changes of nuclear shapes were dependent on abnormal mitoses, which occurred more frequently in the G2 to M phases. Therefore it is suggested that the G2 block phenomenon plays an important role in radiation-induced cell death because the process of cell death by irradiation has been shown to proceed via these abnormal mitoses.
利用移植于裸鼠的人子宫颈癌细胞和小鼠白血病L5178Y细胞，通过流式细胞术（FCM）观察照射后细胞周期的变化，并通过FCM测量照射过程中细胞核的变化。体内和体外实验均导致细胞在G2至M期群体中积累，如通过DNA分布的FCM分析所揭示的那样，导致所谓的G2期阻滞现象。辐射诱导的核形态变化取决于异常有丝分裂，其在G2至M期更频繁地发生。因此，有人提出G2期阻滞现象在辐射诱导的细胞死亡中起重要作用，因为已经表明辐射诱导的细胞死亡过程是通过这些异常有丝分裂进行的。
