Cao S L
Cancer Hospital, Shanghai Medical University.
Zhonghua Zhong Liu Za Zhi. 1990 Jan;12(1):12-4.
Cell kinetics in human hepatoma xenograft in nude mice after gamma irradiation was studied using flow cytometry (FCM) method and the changes of AFP in the xenograft were measured by radioimmunological assay. After 10 Gy irradiation, a marked tumor growth delay for 10 days was observed. Cell cycle analysis revealed an acute but temporary block of cell cycling at G2. About 58% cells were in the G2 phase lasting for 90 hours post-irradiation. A concomitant decrease in serum AFP determined by RIA was also observed. The results indicate that the human hepatoma was quite radio-sensitive.
采用流式细胞术（FCM）研究了γ射线照射后裸鼠人肝癌异种移植瘤的细胞动力学，并通过放射免疫测定法检测了异种移植瘤中甲胎蛋白（AFP）的变化。照射10 Gy后，观察到肿瘤生长明显延迟10天。细胞周期分析显示，细胞周期在G2期出现急性但短暂的阻滞。照射后约58%的细胞处于G2期，持续90小时。同时通过放射免疫分析还观察到血清AFP降低。结果表明，人肝癌对放疗相当敏感。
