Rutgers D H, van Oostrum I E, Noorman van der Dussen M F, Wils I S
Department of Radiotherapy, University Hospital, Utrecht, The Netherlands.
Anal Cell Pathol. 1989 Feb;1(1):53-62.
Monitoring the radiation-induced G2-arrest with flow cytometry disclosed the relationship between the pattern of accumulation in G2 and the cell kinetics (Tc), as assessed with semi-continuous labelling. A close relationship between these two parameters was observed in a murine mammary tumour after high dose-rate irradiation and in human cervix tumours (xenografted and in patients) after low dose-rate irradiation. In inoperable tumours of the oral cavity, a G2-arrest, observed in the first two weeks of fractionated therapy, was found to predict a local cure. Consequently, it was concluded that the cells accumulated in G2 were not doomed cells. This process of radiation-induced synchronization might be exploited in fractionation schemes of which treatment intervals are adjusted to the Tc-related timing of the radiation-induced G2-arrest.
通过流式细胞术监测辐射诱导的G2期阻滞，揭示了G2期积累模式与细胞动力学（Tc）之间的关系，细胞动力学通过半连续标记进行评估。在高剂量率照射后的小鼠乳腺肿瘤以及低剂量率照射后的人宫颈肿瘤（异种移植瘤和患者体内肿瘤）中，观察到这两个参数之间存在密切关系。在口腔无法手术切除的肿瘤中，在分割治疗的前两周观察到的G2期阻滞被发现可预测局部治愈情况。因此，得出的结论是，积累在G2期的细胞并非注定死亡的细胞。这种辐射诱导同步化的过程可应用于分割治疗方案，其中治疗间隔可根据与Tc相关的辐射诱导G2期阻滞时间进行调整。
