Barker D E, Jacobs A G
Ann Clin Biochem. 1982 Mar;19(Pt 2):120-4. doi: 10.1177/000456328201900212.
It is now well established that although acetylsalicylate does not interfere with the commonly used nitration method for measuring paracetamol, salicylate does interfere. Several methods have been used to remove this interference, by solvent extraction, or to make allowance for the interference, for example, by arithmetical correction. We describe a new approach to the problem by modifying the reaction conditions in an attempt to find the optimum conditions for the nitration of paracetamol while at the same time minimising salicylate interference. A method is proposed in which the concentration of nitrite is reduced and a time delay is introduced after the addition of sulphamic acid. This method is shown to be accurate, precise, and linear and exhibits minimal interference from salicylate.
现已充分证实，虽然乙酰水杨酸不会干扰常用的对乙酰氨基酚硝化测定法，但水杨酸会产生干扰。已经采用了几种方法来消除这种干扰，如溶剂萃取法，或者考虑这种干扰，例如通过算术校正法。我们描述了一种解决该问题的新方法，即通过改变反应条件，试图找到对乙酰氨基酚硝化的最佳条件，同时尽量减少水杨酸的干扰。本文提出了一种方法，即降低亚硝酸盐浓度，并在加入氨基磺酸后引入一个时间延迟。结果表明，该方法准确、精密且呈线性，并且水杨酸的干扰极小。
