Longlands M G, Wiener K
Ann Clin Biochem. 1982 May;19(3):187-90. doi: 10.1177/000456328201900312.
The Glynn and Kendal technique for plasma paracetamol assay was investigated in an attempt to eliminate its susceptibility to interference from salicylate. Modification of the nitrating reaction so as to produce milder conditions by omission of hydrochloric acid, reduction of nitrite concentration, and limiting the time of reaction to 2 minutes resulted in far less interference. A further improvement was achieved by reading at 450 nm. The proposed modification reduces the interference from a salicylate concentration of 100 mg/dl to the equivalent paracetamol concentration of 12 mg/l, compared with about 70 mg/l in the unmodified method.
对格林和肯德尔血浆扑热息痛测定技术进行了研究，试图消除其对水杨酸盐干扰的敏感性。通过省略盐酸、降低亚硝酸盐浓度以及将反应时间限制在2分钟来改变硝化反应，以产生更温和的条件，从而使干扰大大减少。通过在450nm处读数实现了进一步的改进。与未改进方法中约70mg/l相比，所提出的改进将水杨酸盐浓度为100mg/dl时的干扰降低至相当于12mg/l的扑热息痛浓度。
