细胞毒性药物诱导的小EMT6球体生长延迟及其在缺氧条件下经米索硝唑预处理后的改变
Growth delay in small EMT6 spheroids induced by cytotoxic drugs and its modification by misonidazole pretreatment under hypoxic conditions.
作者信息
Twentyman P R
出版信息
Br J Cancer. 1982 Apr;45(4):565-70. doi: 10.1038/bjc.1982.93.
Abstract
Experiments have been carried out to determine whether the growth delay induced in small EMT6 spheroids (approximately 250 micrometers in diameter) by a range of cytotoxic drugs can be increased by pre-incubation of the spheroids under hypoxic conditions with misonidazole (MISO). Hypoxic pre-incubation was for 3 or 5 h in the presence of 5 mM MISO, and caused a growth delay of about 1 day or 2 days respectively. Sensitization to nitrogen mustard (HN2), melphalan, chlorambucil, BCNU and CCNU was seen, but the shapes of the dose-response curves and the ratio of effects for 3 h and 5 h pretreatment varied between the drugs. In contrast to the other agents, hypoxic pre-incubation with MISO reduced the spheroid response to adriamycin.
摘要
已经开展了实验，以确定在一系列细胞毒性药物诱导下，小型EMT6球体（直径约250微米）生长延迟是否可以通过在缺氧条件下用米索硝唑（MISO）预孵育球体来增强。在5 mM MISO存在下进行缺氧预孵育3或5小时，分别导致约1天或2天的生长延迟。观察到对氮芥（HN2）、美法仑、苯丁酸氮芥、卡莫司汀和洛莫司汀有增敏作用，但不同药物的剂量反应曲线形状以及3小时和5小时预处理的效应比有所不同。与其他药物相反，用MISO进行缺氧预孵育降低了球体对阿霉素的反应。
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本文引用的文献
1
Enhancement of antitumor activity of alkylating agents by the radiation sensitizer misonidazole.放射增敏剂米索硝唑增强烷化剂的抗肿瘤活性
Cancer Res. 1980 Nov;40(11):4165-72.
2
In vivo interaction of anti-cancer drugs with misonidazole or metronidazole: methotrexate, 5-fluorouracil and adriamycin.抗癌药物与米索硝唑或甲硝唑在体内的相互作用：甲氨蝶呤、5-氟尿嘧啶和阿霉素。
Br J Cancer. 1980 Dec;42(6):861-70. doi: 10.1038/bjc.1980.334.
3
The response of EMT6 tumor spheroids to combined treatment with misonidazole and either nitrogen mustard, adriamycin, or BCNU.EMT6肿瘤球体对米索硝唑与氮芥、阿霉素或卡氮芥联合治疗的反应。
Cancer Clin Trials. 1980 Fall;3(3):253-6.
4
Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival.通过生长延迟和细胞存活来衡量EMT6球体对化疗的反应。
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5
The interaction of misonidazole with radiation, chemotherapeutic agents, or heat: a preliminary report.灭滴灵与辐射、化疗药物或热的相互作用：初步报告。
Cancer Clin Trials. 1980 Fall;3(3):231-6.
6
Studies on the toxicity and radiosensitizing ability of misonidazole under conditions of prolonged incubation.米索硝唑在长时间孵育条件下的毒性及放射增敏能力研究
Radiat Res. 1978 Sep;75(3):541-55.
7
Cell-cycle inhibition by misonidazole of human cells cultivated in vitro under aerobic conditions.在有氧条件下体外培养的人细胞中，米索硝唑对细胞周期的抑制作用。
Br J Cancer. 1979 Nov;40(5):755-60. doi: 10.1038/bjc.1979.257.
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