通过生长延迟和细胞存活来衡量EMT6球体对化疗的反应。
Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival.
作者信息
Twentyman P R
出版信息
Br J Cancer. 1980 Aug;42(2):297-304. doi: 10.1038/bjc.1980.230.
Abstract
Multicellular tumour spheroids of the EMT6 mouse tumour line have been grown in a static (i.e. non-spinner) culture system to a mean spheroid diameter of 250 mum. Samples of spheroids were then exposed for 1 h to graded concentrations of various cytotoxic drugs, and the response assayed by both growth delay and survival of clonogenic cells. For nitrogen mustard (HN2), melphalan, BCNU, CCNU and cis-platinum, a considerable recovery in measured cell survival was seen if correlation between growth delay and cell survival (measured at 24 h) was observed for these 5 agents. For adriamycin, actinomycin D and 5-fluorouracil, no increase in measured cell survival was seen for a 24h delay in assay, and these agents produced longer growth delays for a given level of cell kill.
摘要
EMT6小鼠肿瘤系的多细胞肿瘤球体在静态（即非旋转）培养系统中生长至平均球体直径为250μm。然后将球体样本暴露于不同浓度梯度的各种细胞毒性药物中1小时，并通过克隆细胞的生长延迟和存活率来测定反应。对于氮芥（HN2）、美法仑、卡莫司汀、洛莫司汀和顺铂，如果观察到这5种药物的生长延迟与细胞存活率（在24小时测量）之间的相关性，则在测量的细胞存活率中会出现相当大的恢复。对于阿霉素、放线菌素D和5-氟尿嘧啶，在检测延迟24小时的情况下，测量的细胞存活率没有增加，并且在给定的细胞杀伤水平下，这些药物产生更长的生长延迟。
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