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[人工脂质-脂蛋白膜的结构与物理化学性质]

[Structure and physico-chemical properties of artificial lipid-proteolipid membranes].

作者信息

Giul'khandanian M Z, Manukian K G

出版信息

Biofizika. 1982 Mar-Apr;27(2):259-63.

PMID:7074148
Abstract

Stable flat lipid-proteolipid membranes were obtained from lipid-proteolipid mixture of cattle brain white matter. Equilibrium values of resistance, surface tension and optical thickness of these membranes in KCl and NaCl solutions were measured in the temperature range 25--50 degrees C. An artificial lipid-proteolipid membrane was shown to comprise a mosaic of bilayer lipid regions and proteolipid nodes occupying about 30% of the surface area and adjacent to the aqueous medium. In relation to ionic strength and temperature of the aqeous medium the resistance of such membranes may vary within 10(5)--10(8) Ohm cm2, whereas tension and optical thickness vary comparatively little. There exist two stationary levels of membrane conductivity, and reversible spontaneous transitions between them at fixed temperature and ionic strength of the aqueous medium may proceed.

摘要

从牛脑白质的脂质-蛋白脂质混合物中获得了稳定的扁平脂质-蛋白脂质膜。在25-50摄氏度的温度范围内,测量了这些膜在氯化钾和氯化钠溶液中的电阻、表面张力和光学厚度的平衡值。结果表明,人工脂质-蛋白脂质膜由双层脂质区域和占表面积约30%且与水介质相邻的蛋白脂质节点组成的镶嵌结构。相对于水介质的离子强度和温度,这种膜的电阻可能在10⁵-10⁸欧姆·平方厘米范围内变化,而张力和光学厚度变化相对较小。存在两个膜电导率的稳定水平,并且在固定温度和水介质离子强度下它们之间可能会发生可逆的自发转变。

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