Cadman A D, Fleming R, Guy R H
Biophys J. 1982 Mar;37(3):569-74.
The diffusion of lysozyme chloride in aqueous solution has been studied at 25 degrees C using the Goüy interferometric technique. The concentration dependence of the diffusion coefficient in water has been measured over the concentration range 1.1599-9.1556 gcm-3 and the results suggest a value of D 25, w at infinite dilution of 5.838 x 10(-6) cm2s-1. The variation in diffusion coefficient with ionic strength has also been considered by following the diffusion of 0.45% lysozyme chloride in a series of potassium chloride solutions. The value of D in 0.15 M KCl has been found to be approximately one quarter of that in water alone an the diffusion coefficient has been shown to increase markedly as the KCl concentration is reduced below 0.05 M. Interpretation of these observations involves consideration of solution electrostatic effects.
在25℃下，使用古伊干涉技术研究了氯化溶菌酶在水溶液中的扩散。在1.1599 - 9.1556 g/cm³的浓度范围内测量了扩散系数与水浓度的关系，结果表明在无限稀释时25℃下水的扩散系数D值为5.838×10⁻⁶ cm²/s。通过跟踪0.45%氯化溶菌酶在一系列氯化钾溶液中的扩散，还研究了扩散系数随离子强度的变化。发现在0.15 M KCl中D的值约为仅在水中时的四分之一，并且当KCl浓度降至0.05 M以下时，扩散系数显著增加。对这些观察结果的解释涉及对溶液静电效应的考虑。
