Stock G B
Biophys J. 1976 May;16(5):535-40. doi: 10.1016/S0006-3495(76)85708-6.
A new method of extracting information about bacterial speeds from photon correlation spectroscopy is presented. This method has the advantage that an estimation of the translational speed distribution is directly varied so as to achieve a best least-squares fit to the experimental autocorrelation function. The theory of spline approximations to continuous functions is briefly outlined. The importance of the previously disregarded diffusional component of bacterial motion is discussed. Experimental data from Salmonella at a low scattering angle is analyzed by this method of spline approximation and the distribution of translational speeds is obtained.
提出了一种从光子相关光谱中提取细菌速度信息的新方法。该方法的优点是直接改变平动速度分布的估计值，以便对实验自相关函数实现最佳最小二乘拟合。简要概述了连续函数的样条逼近理论。讨论了先前被忽视的细菌运动扩散分量的重要性。用这种样条逼近方法分析了低散射角下沙门氏菌的实验数据，并得到了平动速度分布。
