Nathanson R M
Can J Comp Med. 1982 Jan;46(1):57-9.
The culture conditions for obtaining the maximum transformation of chicken lymphocytes stimulated by the mitogen Concanavalin A are described in this paper. Lymphocytes were cultured in round bottom microtiter tissue culture plates and the incorporation of 3H-methyl-thymidine (3H-TdR) into lymphocyte deoxyribonucleic acid was used to quantitatively evaluate transformation. Maximum incorporation of 3H-methyl-thymidine occurred when 2 x 10(6) lymphocytes and 4 micrograms of Concanavalin A were added to each culture. Deoxyribonucleic acid synthesis was greatest during the period of 18 to 36 hours after addition of mitogen. Differences between chicken and mammalian lymphocyte cultures are discussed.
本文描述了获得由促细胞分裂剂刀豆球蛋白A刺激的鸡淋巴细胞最大转化率的培养条件。淋巴细胞在圆底微量滴定组织培养板中培养，并且将3H-甲基胸腺嘧啶核苷（3H-TdR）掺入淋巴细胞脱氧核糖核酸中用于定量评估转化。当向每个培养物中加入2×10(6)个淋巴细胞和4微克刀豆球蛋白A时，3H-甲基胸腺嘧啶核苷的掺入量最大。在加入促细胞分裂剂后的18至36小时期间，脱氧核糖核酸合成最为旺盛。讨论了鸡和哺乳动物淋巴细胞培养之间的差异。
