Panko W B, Mattioli C A, Wheeler T M
Cancer. 1982 May 15;49(10):2148-52. doi: 10.1002/1097-0142(19820515)49:10<2148::aid-cncr2820491027>3.0.co;2-9.
Forty-nine specimens of carcinoma of the breast were analyzed for estrogen receptor content using the sucrose gradient method and a histochemical method using a conjugate of estradiol (6-BSA-Fluor-CMO-17 beta-estradiol). The specimens analyzed by the histochemical method were graded for fluorescent intensity, percentage of cells fluorescing, and cellularity. Only 53.1% (26/49) of the specimens were classified into the same three categories (positive, negative and ambiguous) as classified by the biochemical assay. If the negative and ambiguous categories were combined, the degree of correspondence rose to only 63.3% (31/49). Because the classification scheme was arbitrary and because the results of the biochemical assay might be correlated with one or more factors, univariate and multivariate analyses were used to examine the data for any underlying relationships. Only the age of the patient was significantly correlated with the results of the biochemical assay. Importantly, neither the percentage of cells fluorescing nor the fluorescent intensity was correlated with the results of the biochemical assay. Terms to account for various interactions of parameters did not enhance the association with the biochemical results. While this histochemical assay may eventually prove to be effective in selecting patients for endocrine therapy, it does not seem to be a substitute for the standard biochemical assay for estrogen receptors presently used.
采用蔗糖梯度法和使用雌二醇结合物（6 - BSA - Fluor - CMO - 17β - 雌二醇）的组织化学方法，对49份乳腺癌标本的雌激素受体含量进行了分析。通过组织化学方法分析的标本，按照荧光强度、荧光细胞百分比和细胞密度进行分级。通过组织化学方法分类的标本中，只有53.1%（26/49）与生化检测分类的同一三类（阳性、阴性和不明确）相符。如果将阴性和不明确类别合并，符合程度也仅升至63.3%（31/49）。由于分类方案具有随意性，且生化检测结果可能与一个或多个因素相关，因此使用单变量和多变量分析来检查数据中是否存在任何潜在关系。只有患者年龄与生化检测结果显著相关。重要的是，荧光细胞百分比和荧光强度均与生化检测结果无关。用于解释参数各种相互作用的术语，并未增强与生化结果的关联。虽然这种组织化学检测最终可能被证明对选择内分泌治疗的患者有效，但它似乎不能替代目前使用的雌激素受体标准生化检测。
