Massey T H, Barta J S
Clin Chem. 1982 May;28(5):1174-6.
Patterns of creatine kinase (CK, EC 2.7.3.2) isoenzymes were studied in apparently healthy one- to 10-day-old neonates, by use of a sensitive fluorescent staining method with Sclavo CK-F/6001 reagent. Mean activities of CK3 (MM, 105 U/L), CK2 (MB, 6.8 U/L), CK1 (BB, 11 U/L), adenylate kinase (EC 2.7.4.3) anodal to CK3, and a fluorescent albumin artifact were found. Pooled plasma from neonates is recommended as a control because it defines the albumin artifact and approximates the activity of CK2 that must be observed after proper staining before a diagnosis of myocardial infarction can be made.
采用Sclavo CK-F/6001试剂的敏感荧光染色法，对1至10日龄看似健康的新生儿的肌酸激酶（CK，EC 2.7.3.2）同工酶模式进行了研究。发现CK3（MM，105 U/L）、CK2（MB，6.8 U/L）、CK1（BB，11 U/L）、位于CK3阳极的腺苷酸激酶（EC 2.7.4.3）以及荧光白蛋白假象的平均活性。建议使用新生儿混合血浆作为对照，因为它可确定白蛋白假象，并接近在做出心肌梗死诊断之前正确染色后必须观察到的CK2活性。
