速率散射比浊免疫分析法检测脐血血清中β-脂蛋白：家族性高胆固醇血症的一种潜在筛查试验
Quantitation of beta-lipoprotein in cord serum by rate nephelometric immunoassay: a potential screening test for familial hypercholesterolemia.
作者信息
Brewster T G, Waite D J, Hudson G A
出版信息
Clin Chem. 1982 May;28(5):1192-6.
We measured beta-lipoprotein in 232 cord sera by rate nephelometry and compared the results with those obtained by electroimmunoassay. The mean (mean +/- two standard deviations) was 544 (293-1009) mg/L by rate nephelometry and 503 (265-955) mg/L by electroimmunoassay. The between-assay correlation coefficient was 0.891. Between-run coefficients of variation were 3.5-4.4% for nephelometry and 11-14% for electroimmunoassay. Nephelometry was faster, more precise, and less labor intensive than electroimmunoassay. For these reasons, rate nephelometry appears to be a more suitable assay system for mass screening, should detection of familial hypercholesterolemia prove feasible and desirable.
我们采用速率散射比浊法测定了232份脐血血清中的β脂蛋白，并将结果与免疫电泳法所得结果进行比较。速率散射比浊法测定的均值（均值±两个标准差）为544（293 - 1009）mg/L，免疫电泳法测定的均值为503（265 - 955）mg/L。两种检测方法之间的相关系数为0.891。速率散射比浊法批间变异系数为3.5% - 4.4%，免疫电泳法批间变异系数为11% - 14%。速率散射比浊法比免疫电泳法速度更快、更精确且劳动强度更低。基于这些原因，如果事实证明检测家族性高胆固醇血症可行且有必要，那么速率散射比浊法似乎是更适合大规模筛查的检测系统。
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