Vladutiu G D, Glueck C J, Schultz M T, McNeely S, Guthrie R
Clin Chem. 1980 Aug;26(9):1285-90.
We describe an electroimmunodiffusion technique for measuring beta-lipoprotein in cord blood spotted on filter paper. A series of cord-blood samples, taken from 916 consecutive live-birth infants, was spotted directly onto filter paper and assayed for beta-lipoprotein. Eleven had above-normal beta-lipoprotein. Of these 11, seven were tested two to six months later, along with their parents, for total cholesterol and triglyceride concentrations. Five infants had increased cholesterol values, and four parents of these infants had either increased cholesterol or triglyceride values. We also measured beta-lipoprotein concentrations in 63 paired samples of dried cord-blood and three-day post-delivery blood specimens, routinely collected for phenylketonuria screening. We saw a significant correlation between results for the specimens, but detected no cases of increased beta-lipoprotein. beta-Lipoprotein assay in dried specimens of cord blood is simple, inexpensive, and potentially is useful in mass screening of newborns for familial type II and combined hyperlipidemia.
我们描述了一种用于测定滤纸上采集的脐血中β脂蛋白的免疫电扩散技术。从916例连续活产婴儿采集的一系列脐血样本，直接点样于滤纸上并检测β脂蛋白。11例β脂蛋白高于正常水平。在这11例中，7例在两至六个月后与其父母一起检测了总胆固醇和甘油三酯浓度。5例婴儿胆固醇值升高，这些婴儿的4位父母胆固醇或甘油三酯值升高。我们还在63对用于苯丙酮尿症筛查而常规采集的干脐血样本和产后三天血液标本中检测了β脂蛋白浓度。我们发现这些标本的检测结果之间存在显著相关性，但未检测到β脂蛋白升高的病例。脐血干标本中的β脂蛋白检测简单、廉价，可能有助于对新生儿进行家族性II型和混合型高脂血症的大规模筛查。
