Shaw W
Clin Chem. 1982 May;28(5):1196-7.
The algebraic equivalency of nine different methods for the linearization of saturation-type radioassay data was previously demonstrated mathematically to hold under the assumption that the antibody is saturated with ligand. Although compete saturation with antibody never occurs, a good approximation to a saturation condition occurs when the product of the labeled ligand and the affinity constant is 100 or more. The mathematical basis for determining when methods for the linearization of saturation-type radioassay data are valid is derived from a development of the first-order mass action law applied to ligand-antibody reactions.
先前已通过数学方法证明，在抗体被配体饱和的假设下，九种不同的饱和型放射分析数据线性化方法的代数等效性成立。尽管抗体与配体的完全饱和情况从未发生，但当标记配体与亲和常数的乘积为100或更大时，会出现接近饱和条件的良好近似值。确定饱和型放射分析数据线性化方法何时有效的数学基础，源自对应用于配体 - 抗体反应的一级质量作用定律的推导。
