Ghielmi S, Pizzoccolo G, Iacobello C, Albertini A, Arosio P
Clin Chim Acta. 1982 Apr 23;120(3):285-94. doi: 10.1016/0009-8981(82)90369-2.
Serum ferritin is normally quantitated by sensitive radio- or enzymoimmunoassays, which are based either on a competitive reaction between the unlabelled and labelled antigen (RIA), or on a non-competitive reaction between the sample and the labelled antibody (IRMA and ELISA). Both methods appear to be satisfactory for the clinical evaluation of serum ferritin. However, the effect of these methods on the quantitation of serum ferritin has never been carefully studied. This paper describes and compares five different immunoassays for the evaluation of serum ferritin: two competitive RIAs, one non-competitive IRMA and two non-competitive ELISAs. The same anti-ferritin antibody and ferritin standards were used for all the assays. The RIAs were found to be less sensitive than either the ELISAs or the IRMA, and all showed a similar degree of cross-reactivity with ferritin extracted from spleen and HeLa cells. A significant difference between the assays was found in the determination of serum ferritin: in fact the RIAs over-estimated serum ferritin by about 50% more than the IRMA, whereas the ELISAs under-estimated serum ferritin by about 15% less than the IRMA.
血清铁蛋白通常通过灵敏的放射免疫分析或酶免疫分析进行定量，这些分析要么基于未标记抗原与标记抗原之间的竞争反应（放射免疫分析），要么基于样品与标记抗体之间的非竞争反应（免疫放射分析和酶联免疫吸附测定）。这两种方法对于血清铁蛋白的临床评估似乎都令人满意。然而，这些方法对血清铁蛋白定量的影响从未得到仔细研究。本文描述并比较了五种不同的用于评估血清铁蛋白的免疫分析方法：两种竞争型放射免疫分析、一种非竞争型免疫放射分析和两种非竞争型酶联免疫吸附测定。所有分析均使用相同的抗铁蛋白抗体和铁蛋白标准品。结果发现，放射免疫分析不如酶联免疫吸附测定或免疫放射分析灵敏，并且所有方法与从脾脏和宫颈癌细胞系提取的铁蛋白都表现出相似程度的交叉反应性。在血清铁蛋白的测定中发现各分析方法之间存在显著差异：实际上，放射免疫分析比免疫放射分析高估血清铁蛋白约50%，而酶联免疫吸附测定比免疫放射分析低估血清铁蛋白约15%。
