一种用于定量血清铁蛋白的固相酶免疫测定法。

A solid phase enzyme immunoassay for the quantitation of serum ferritin.

作者信息

Lee M, Burgett M W

出版信息

Clin Chim Acta. 1981 May 5;112(2):241-6. doi: 10.1016/0009-8981(81)90383-1.

Abstract

A non-competitive solid phase enzyme immunoassay for the measurement of ferritin in human serum is described. This procedure involves the use of specific antibody covalently attached to derivatized hydrophilic microparticles and enzyme labeled specific antibody. Reproducible results were achieved within 4 h for ferritin in serum in the range of 4 ng/ml to 250 ng/ml. Ferritin levels as low as 0.4 ng/ml can be measured. The enzyme immunoassay and three commercially available radioimmunoassay(RIA) kits were used to determine serum ferritin levels in healthy adults. Good agreement was found between the enzyme immunoassay and the RIA methods.

摘要

本文描述了一种用于测定人血清中铁蛋白的非竞争性固相酶免疫测定法。该方法涉及使用共价连接到衍生化亲水性微粒上的特异性抗体和酶标记的特异性抗体。对于血清中铁蛋白浓度在4 ng/ml至250 ng/ml范围内的样本，可在4小时内获得可重复的结果。最低可检测到0.4 ng/ml的铁蛋白水平。使用该酶免疫测定法和三种市售放射免疫测定（RIA）试剂盒来测定健康成年人的血清铁蛋白水平。发现酶免疫测定法和RIA方法之间具有良好的一致性。