Froese A, Helm R M, Conrad D H, Isersky C, Ishizaka T
Immunology. 1982 May;46(1):117-23.
Receptors for IgE of rat basophilic leukaemia (RBL) cells, maintained in different laboratories were isolated by means of various anti-receptor antisera, and characterized by sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis. Most antisera were shown to react with a receptor designated H and which, as shown previously, could be isolated by IgE-Sepharose but not by a combination of IgE and anti-IgE. Only two highly purified anti-receptor antibody preparations, which had been purified by adsorption to and elution from rat basophilic leukaemia cells, reacted primarily with a second kind of receptor molecule which had previously been designated R. Some indirect evidence was obtained which suggests that H is a highly immunogenic molecule. It was confirmed that the apparent molecular weight of H-like molecules varied on different RBL cell lines.
用不同抗受体抗血清分离在不同实验室培养的大鼠嗜碱性白血病（RBL）细胞的IgE受体，并通过十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳进行特性分析。多数抗血清显示与一种名为H的受体发生反应，如先前所示，该受体可通过IgE-琼脂糖凝胶分离，但不能通过IgE与抗IgE的组合分离。只有两种经大鼠嗜碱性白血病细胞吸附和洗脱纯化的高度纯化抗受体抗体制剂，主要与另一种先前命名为R的受体分子发生反应。获得了一些间接证据，表明H是一种高度免疫原性的分子。证实了不同RBL细胞系上H样分子的表观分子量有所不同。
