Hayashi H, Miyao M, Morita S
J Biochem. 1982 Mar;91(3):1017-27. doi: 10.1093/oxfordjournals.jbchem.a133751.
Bacteriochlorophyll (Bchl)-protein complexes were isolated from Rhodopseudomonas palustris. Detergent treatment and polyacrylamide gel electrophoresis under rather mild conditions resulted in clear separation of light-harvesting Bchls into B870 Bhls and B800-850 Bchls as two distinctly different protein complexes. It was shown that almost all the Bchl contained in intracytoplasmic membranes was recovered as either B870-reaction center complexes or B800-850 complexes without loss of Bchls as free pigments. Furthermore, it was shown that the spectral form of each type of Bchl was not altered by this procedure for isolation, as judged by the calculated absorption spectrum which was the sum of the absorption spectra of the isolated complexes. Fluorescence measurement of the isolated complexes and intracytoplasmic membranes not only supported these ideas, but also indicated that the excited state was actually transferred from B800-850 Bchls to B870 Bchls. The isolation and measurements of absorption and fluorescence were carried out on spectrally different types of intracytoplasmic membrane from cells cultured under different culture conditions. The B870-reaction center complexes from different types of intracytoplasmic membrane were spectrally identical. The B800-850 complexes were widely different in absorption spectra, especially in the ratios of peaks at 800 and 850 nm, but exhibited similar fluorescence spectra.
从沼泽红假单胞菌中分离出细菌叶绿素（Bchl）-蛋白质复合物。在相当温和的条件下进行去污剂处理和聚丙烯酰胺凝胶电泳，可将捕光Bchls清晰地分离为B870 Bhls和B800 - 850 Bchls这两种明显不同的蛋白质复合物。结果表明，胞内膜中所含的几乎所有Bchl都以B870 - 反应中心复合物或B800 - 850复合物的形式回收，没有游离色素形式的Bchl损失。此外，根据分离复合物吸收光谱之和计算出的吸收光谱判断，该分离过程不会改变每种类型Bchl的光谱形式。对分离复合物和胞内膜进行荧光测量，不仅支持了这些观点，还表明激发态实际上从B800 - 850 Bchls转移到了B870 Bchls。对在不同培养条件下培养的细胞中光谱不同类型的胞内膜进行了吸收和荧光的分离及测量。不同类型胞内膜的B870 - 反应中心复合物光谱相同。B800 - 850复合物的吸收光谱差异很大，尤其是在800和850 nm处的峰比值，但荧光光谱相似。
