Rehman A, Gates S C, Webb J W
J Chromatogr. 1982 Mar 12;228:103-12. doi: 10.1016/s0378-4347(00)80423-5.
Four methods for extracting organic acids from human urine prior to analysis by high-performance liquid chromatography (HPLC) were compared. The methods were manual solvent extraction with ethyl acetate and diethyl ether, continuous solvent extraction, anion exchange with pyridinium acetate as the eluting solvent and anion exchange with hydrochloric acid as the eluting solvent. All four methods produced samples that could be analyzed by reversed-phase HPLC, but the continuous solvent extraction and anion exchange with pyridinium acetate methods gave the best reproducibilities (approximately 6% relative standard deviations). Pretreatment of the urine with barium hydroxide and hydroxylamine hydrochloride prior to anion exchange did not markedly alter the HPLC profiles.
比较了在通过高效液相色谱法（HPLC）分析之前从人尿中提取有机酸的四种方法。这些方法分别是用乙酸乙酯和乙醚进行手动溶剂萃取、连续溶剂萃取、以乙酸吡啶鎓作为洗脱溶剂的阴离子交换法以及以盐酸作为洗脱溶剂的阴离子交换法。所有这四种方法所得到的样品都能够通过反相HPLC进行分析，但连续溶剂萃取法和以乙酸吡啶鎓进行阴离子交换的方法具有最佳的重现性（相对标准偏差约为6%）。在进行阴离子交换之前用氢氧化钡和盐酸羟胺对尿液进行预处理并没有显著改变HPLC图谱。
