Boujet C, Drouet C, Decoux G, Favier A
J Chromatogr. 1982 Mar 12;228:67-74.
Ten organic acids are extracted from urine. Two extraction methods are used: anion exchange on DEAE-Sephadex columns and organic solvent extraction with five different solvents: diethyl ether, ethyl acetate, isopropyl chloride, light petroleum, and tetrahydrofuran. In order to quantify the extractions, the corresponding 14C-labeled acids are added to standard acid solutions and extraction rates are measured by a liquid scintillation counting system. The results show that: (1) The efficiency of anion exchange is generally good for all tested acids. (2) The extraction efficiency is not identical for the different solvents, one solvent being more efficient for a certain acid than another: tetrahydrofuran, which is generally a good solvent, is too hygroscopic to be usable. Isopropyl chloride and light petroleum are too specific with the most apolar molecules. Ethyl acetate and diethyl ether are similar and usable because of their acceptable solubilisation power as to the most polar molecules, their good solubilisation reproducibility and their readiness of use. (3) The solvent extraction method is not as time-consuming as the anion-exchange method which generally requires lengthy elution and extraction.
从尿液中提取出10种有机酸。采用了两种提取方法：一是在DEAE - 葡聚糖凝胶柱上进行阴离子交换，二是用5种不同溶剂进行有机溶剂萃取，这5种溶剂分别是：乙醚、乙酸乙酯、异丙基氯、轻质石油和四氢呋喃。为了对提取物进行定量分析，将相应的14C标记酸添加到标准酸溶液中，并通过液体闪烁计数系统测量提取率。结果表明：（1）阴离子交换效率对于所有测试酸来说总体良好。（2）不同溶剂的萃取效率不尽相同，一种溶剂对某一种酸的萃取效率高于另一种：四氢呋喃通常是一种良好的溶剂，但因其吸湿性太强而无法使用。异丙基氯和轻质石油对极性最小的分子选择性过高。乙酸乙酯和乙醚相似且可用，因为它们对极性最大的分子具有可接受的溶解能力、良好的溶解重现性且易于使用。（3）溶剂萃取法不像阴离子交换法那样耗时，阴离子交换法通常需要长时间的洗脱和萃取。
