McCoshen J A, Tomita K, Fernandez C, Tyson J E
J Clin Endocrinol Metab. 1982 Jul;55(1):166-9. doi: 10.1210/jcem-55-1-166.
The presence of PRL in high concentration in human amniotic fluid has been related to changes in water transport across amnion but not chorion leave. The cellular composition of human amniotic epithelium has been reported to include at least two structurally distinct cell types, known as light cells and dark cells, that differ in their ability to transport large molecules. In the present study, human amnion obtained from term cesarean section was evaluated through the use of autoradiography as to its ability to localize hormones of similar and variant molecular sizes. Amniotic epithelium exposed to [125I]human PRL, [125I]human Gh, [125I]human beta-endorphin, [125I]bovine FSH, and sodium 125I alone was found to display a selectivity in its ability to localize [125I]human PRL only. Furthermore, the selective localization of [125I]human PRL was found to be specific to the light cells, because dark cells failed to localize any of the other tracers used. These data provide additional evidence in support of the recently proposed concept that term human amniotic epithelium consists of at least two functionally distinct cell types. Furthermore, the high levels of PRL in amniotic fluid may have a specific physiologic role in the amniotic membrane during human gestation.
人羊水中高浓度催乳素（PRL）的存在与羊水而非绒毛膜跨膜水转运的变化有关。据报道，人羊膜上皮的细胞组成至少包括两种结构不同的细胞类型，即亮细胞和暗细胞，它们在运输大分子的能力上有所不同。在本研究中，通过放射自显影术评估了从足月剖宫产获取的人羊膜对分子大小相似和不同的激素进行定位的能力。发现暴露于[125I]人PRL、[125I]人生长激素（Gh）、[125I]人β-内啡肽、[125I]牛促卵泡激素（FSH）和单独的125I钠的羊膜上皮仅对[125I]人PRL具有定位选择性。此外，发现[125I]人PRL的选择性定位对亮细胞具有特异性，因为暗细胞未能定位所使用的任何其他示踪剂。这些数据为最近提出的足月人羊膜上皮由至少两种功能不同的细胞类型组成这一概念提供了额外的证据。此外，羊水中高水平的PRL可能在人类妊娠期间的羊膜中具有特定的生理作用。
