Nakayama S, Mold C, Gewurz H, du Clos T W
J Immunol. 1982 Jun;128(6):2435-8.
The capacity of CRP to alter the clearance and site of sequestration of erythrocytes was examined. Mouse erythrocytes, coated with PnC to provide a binding site for CRP, were radiolabeled and injected into homologous mice. The liver, spleen, kidneys, and lungs were removed and counted to assess the site of erythrocyte clearance. In the presence of CRP, an increase in splenic sequestration of E-PnC was observed along with a slight decrease in hepatic sequestration. This altered clearance pattern was dependent on both C activation and on the continued presence of CRP. The clearance of cells coated with IgM or IgG antibody to PnC was compared. In both cases, antibody caused an increase in sequestration by the spleen. However, IgM-coated cells required C for splenic clearance, whereas IgG-coated cells did not. We have shown in these experiments that CRP can affect the in vivo pattern of organ sequestration of cells to which it is bound in a manner similar to antibody.
研究了CRP改变红细胞清除和隔离部位的能力。用肺炎球菌C多糖（PnC）包被小鼠红细胞以提供CRP的结合位点，对其进行放射性标记后注射到同系小鼠体内。取出肝脏、脾脏、肾脏和肺并计数，以评估红细胞清除部位。在存在CRP的情况下，观察到脾脏对包被PnC的红细胞（E-PnC）的隔离增加，同时肝脏隔离略有减少。这种改变的清除模式既依赖于补体（C）激活，也依赖于CRP的持续存在。比较了用抗PnC的IgM或IgG抗体包被的细胞的清除情况。在这两种情况下，抗体均导致脾脏隔离增加。然而，包被IgM的细胞需要补体进行脾脏清除，而包被IgG的细胞则不需要。我们在这些实验中表明，CRP能够以类似于抗体的方式影响其结合细胞在体内的器官隔离模式。
