Schlaeger R, Röhr A
J Clin Chem Clin Biochem. 1982 Mar;20(3):147-50. doi: 10.1515/cclm.1982.20.3.147.
A photometric method for chymotrypsin is proposed, using the substrate N-acetyl-L-tyrosine ethyl ester, which is already used in the titrimetric procedure. Hydrolysis of the ester bond produces equal amounts of ethanol and acetyltyrosine, the latter being measured in the titrimetric method. The ethanol can easily be measured by the alcohol dehydrogenase method in the trichloroacetic acid supernatant. Suitable test conditions are reported.
提出了一种用于胰凝乳蛋白酶的光度法，使用底物N-乙酰-L-酪氨酸乙酯，该底物已用于滴定法中。酯键的水解产生等量的乙醇和乙酰酪氨酸，后者在滴定法中进行测量。乙醇可以很容易地通过酒精脱氢酶法在三氯乙酸上清液中进行测量。报告了合适的测试条件。
