Fau D, Chanez M, Bois-Joyeux B, Delhomme B, Peret J
J Nutr. 1982 May;112(5):833-40. doi: 10.1093/jn/112.5.833.
Adult male rats were force-fed either a single dose of 200 mg of DL-methionine or an amino acid mixture with the pattern of casein. The animals were anesthetized with pentobarbital at 0, 1.5, 3 and 4 hours after intubation, and samples of liver were removed by a freeze-clamping technique at 0, 15 and 30 seconds of ischemia. Hepatic ATP, ADP, AMP, Pi, glucose-6-phosphate, glucose and certain nitrogenous compounds as well as plasma glucose were determined. After methionine was given by intubation, hepatic methionine and glutathione increased severalfold within 1.5 hours; cystathionine showed a transient rise at this time but returned to normal at 3 and 4 hours, when taurine was progressively increasing. Several nonessential amino acids decreased, suggesting that they may be utilized for energy. Methionine force-feeding did not modify the concentration of hepatic adenine nucleotides and probably did not change their turnover, as estimated from changes during ischemia. The level and production of Pi during ischemia was increased however. After force-feeding the amino acid mixture, hepatic methionine, cystathionine and taurine were unaffected, and glutathione increased only at hours 3 and 4; glycine and threonine were elevated by 1.5 hours. Hepatic adenine nucleotides, inorganic phosphate and glucose were not significantly affected by the feeding of amino acids by intubation.
成年雄性大鼠被强制喂食单剂量200毫克的DL-蛋氨酸或具有酪蛋白模式的氨基酸混合物。在插管后0、1.5、3和4小时，用戊巴比妥麻醉动物，并在缺血0、15和30秒时通过冷冻钳夹技术取出肝脏样本。测定肝脏中的三磷酸腺苷（ATP）、二磷酸腺苷（ADP）、一磷酸腺苷（AMP）、无机磷酸盐（Pi）、6-磷酸葡萄糖、葡萄糖和某些含氮化合物以及血浆葡萄糖。经插管给予蛋氨酸后，肝脏中的蛋氨酸和谷胱甘肽在1.5小时内增加了几倍；此时胱硫醚短暂升高，但在3和4小时恢复正常，此时牛磺酸逐渐增加。几种非必需氨基酸减少，表明它们可能被用于提供能量。从缺血期间的变化估计，强制喂食蛋氨酸并未改变肝脏腺嘌呤核苷酸的浓度，可能也未改变其周转率。然而，缺血期间Pi的水平和生成增加。在强制喂食氨基酸混合物后，肝脏中的蛋氨酸、胱硫醚和牛磺酸未受影响，谷胱甘肽仅在3和4小时增加；甘氨酸和苏氨酸在1.5小时时升高。插管喂食氨基酸对肝脏腺嘌呤核苷酸、无机磷酸盐和葡萄糖没有显著影响。
