组蛋白H3和H4四聚体的结晶。
Crystallization of the tetramer of histones H3 and H4.
作者信息
Lattman E, Burlingame R, Hatch C, Moudrianakis E N
出版信息
Science. 1982 May 28;216(4549):1016-8. doi: 10.1126/science.7079748.
PMID:7079748
Abstract
Crystals of the histone tetramer (H3-H4)2 from calf thymus have been grown. The crystals yield x-ray diffraction patterns with Bragg spacings as small as 3.5 angstroms. Crystals grown from two types of preparations have the symmetry of the space group P61 (or P65). The best crystals were grown from histones that had the amino terminal arms removed by mild trypsinization.
摘要
已培养出小牛胸腺组蛋白四聚体（H3 - H4）2的晶体。这些晶体产生的X射线衍射图谱的布拉格间距小至3.5埃。从两种不同制备物中培养出的晶体具有空间群P61（或P65）的对称性。最好的晶体是由经温和胰蛋白酶消化去除了氨基末端臂的组蛋白培养而成的。
Zotero 插件