血清中氯霉素的高效液相色谱测定法
High-performance liquid chromatographic assay of chloramphenicol in serum.
作者信息
Koup J R, Brodsky B, Lau A, Beam T R
出版信息
Antimicrob Agents Chemother. 1978 Sep;14(3):439-43. doi: 10.1128/AAC.14.3.439.
Abstract
A new method for the analysis of serum chloramphenicol by reversed-phase, high-performance liquid chromatography (HPLC) is described. The method involves a preliminary extraction of 0.1 ml of serum with ethyl acetate containing an internal standard, chromatography with a reversed-phase C18 microparticulate column with an acetonitrile-acetate buffer mobile phase, and detection by measuring UV absorbance at 270 nm. Assay performance was compared with an existing microbiological assay. The HPLC method demonstrated both increased precision and increased sensitivity. The specificity of the HPLC method was also evaluated. The new method presents an alternative approach to the analysis of clinical specimens.
摘要
本文描述了一种采用反相高效液相色谱法（HPLC）分析血清中氯霉素的新方法。该方法包括用含有内标的乙酸乙酯对0.1 ml血清进行初步萃取，采用反相C18微粒柱、以乙腈 - 醋酸缓冲液为流动相进行色谱分析，并通过在270 nm处测量紫外吸光度进行检测。将该分析方法的性能与现有的微生物测定法进行了比较。HPLC法显示出更高的精密度和灵敏度。还评估了HPLC法的特异性。该新方法为临床标本分析提供了一种替代方法。
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