Belous A M, Petrenko A Iu
Ukr Biokhim Zh (1978). 1982 Mar-Apr;54(2):194-7.
The calcium-transport function of the rat liver mitochondria was studied as affected by the freezing and thawing rates. It is established that the active Ca2+ transport system is disturbed in the process of freezing-thawing. An extent of this system damage depends mainly in the sample thawing rate. Mitochondria frozen at any rate but thawed at a slow rate become practically unable to transport Ca2+. The fast and slow freezing and fast thawing decrease the Ca2+ accumulation rate and the "calcium capacity" of mitochondria and promote and untimely release of accumulated Ca2+. Disturbances of the mitochondria calcium-transporting function is due to an increase in the proton conductivity of the mitochondria internal membrane.
研究了冻融速率对大鼠肝脏线粒体钙转运功能的影响。结果表明，活性Ca2+转运系统在冻融过程中受到干扰。该系统受损的程度主要取决于样品的解冻速率。以任何速率冷冻但以缓慢速率解冻的线粒体实际上变得无法转运Ca2+。快速和慢速冷冻以及快速解冻会降低线粒体的Ca2+积累速率和“钙容量”，并促进积累的Ca2+过早释放。线粒体钙转运功能的紊乱是由于线粒体内膜质子传导率增加所致。
