Suzuki O, Matsumoto T, Katsumata Y, Oya M
Z Rechtsmed. 1982;88(1-2):67-73. doi: 10.1007/BF00200737.
A new enzymatic method for the quantitation of spermine in human semen, based on the specific reaction of barley seedling polyamine oxidase with spermine, is described. Small amounts of human semen were incubated with the polyamine oxidase; hydrogen peroxide formed in the oxidase reaction was measured photometrically by coupling 4-aminoantipyrine with N-ethyl-N-(3-methylphenyl)-N'-acetylethylenediamine in the presence of peroxidase. The detection limit of spermine of this method was about 10 nmol per tube. The mean level of spermine in human semen was 2.41 mumol/ml; the levels in vaginal fluid, saliva, serum, and urine were below the detectable limit by this procedure.
本文描述了一种基于大麦幼苗多胺氧化酶与精胺的特异性反应来定量测定人类精液中精胺的新酶法。将少量人类精液与多胺氧化酶一起孵育；通过在过氧化物酶存在下将4-氨基安替比林与N-乙基-N-(3-甲基苯基)-N'-乙酰乙二胺偶联，光度法测定氧化酶反应中形成的过氧化氢。该方法精胺的检测限约为每管10 nmol。人类精液中精胺的平均水平为2.41 μmol/ml；阴道液、唾液、血清和尿液中的水平低于该方法的检测限。
