一种使用人胎盘二胺氧化酶的人尿中总二胺的荧光测定法。

A fluorometric assay for total diamines in human urine using human placental diamine oxidase.

作者信息

Matsumoto T, Suzuki O, Katsumata Y, Oya M, Nimura Y, Akita M, Hattori T

出版信息

Clin Chim Acta. 1981 May 5;112(2):141-8. doi: 10.1016/0009-8981(81)90372-7.

Abstract

The detailed procedure for a new fluorometric assay for total diamines in human urine is described. The diamines were purified from the urine by cation-exchange chromatography and incubated with human placental diamine oxidase. Hydrogen peroxide formed in the diamine oxidase reaction was measured fluorometrically by converting homovanillic acid to a highly fluorescent compound in the presence of peroxidase. Because of its simplicity and high sensitivity, our present method seems useful for routine clinical investigation. The data obtained from normal subjects and patients suffering from various forms of cancer are also presented.

摘要

本文描述了一种用于检测人尿中总二胺的新型荧光测定法的详细步骤。通过阳离子交换色谱法从尿液中纯化二胺，并与人胎盘二胺氧化酶一起孵育。在过氧化物酶存在的情况下，通过将高香草酸转化为高荧光化合物，荧光法测定二胺氧化酶反应中形成的过氧化氢。由于其简单性和高灵敏度，我们目前的方法似乎可用于常规临床研究。还展示了从正常受试者和患有各种癌症的患者那里获得的数据。