Simultaneous fluorometric assay of histamine and histidine in biological fluid using an automated analyser.

A sensitive fluorometric method for the assay of histamine and histidine was developed. The method allows for the automated simultaneous assay of the release of these substances from tissue and blood cell preparations in immune and non-immune reactions. The sensitivity for histamine and histidine were 0.5 ng and 10 ng per ml respectively. The assay procedure for histidine, however, was not as specific as that for histamine.