Degtiar' V G
Biokhimiia. 1982 Apr;47(4):587-93.
Evidence for specific binding of [3H]5 alpha-androstane-3 beta, 17 beta-diol (3 beta-diol) in the cytosol of male and female rats was confirmed by determining the apparent dissociation constant and the number of binding sites calculated by the Scatchard method. Centrifugation of hypophysial cytosols in sucrose density gradient revealed the binding at 4.5 S and 8--8.5 S and specific binding of 3 beta-diol around 4.5 S. The presence of protein-bound radioactivity was shown by gel-filtration of Sephadex G-200. The specific binding of the protein was demonstrated in the region of bovine serum albumin elution. Data on the effect of some androgenes and estrogenes on the specific binding of 3 beta-diol by the cytosol of male and female rat hypophysis are presented.
通过用Scatchard法测定表观解离常数和结合位点数量，证实了雄性和雌性大鼠胞质溶胶中存在[3H]5α-雄甾烷-3β,17β-二醇（3β-二醇）的特异性结合。垂体胞质溶胶在蔗糖密度梯度中离心显示在4.5 S和8 - 8.5 S处有结合，且3β-二醇在4.5 S左右有特异性结合。通过Sephadex G - 200凝胶过滤显示存在蛋白质结合的放射性。在牛血清白蛋白洗脱区域证实了该蛋白质的特异性结合。给出了一些雄激素和雌激素对雄性和雌性大鼠垂体胞质溶胶中3β-二醇特异性结合影响的数据。
