Bentzen C L, Acuff K J, Marechal B, Rosenthal M A, Volk M E
Clin Chem. 1982 Jul;28(7):1451-6.
A quick and practical procedure based upon the principles of affinity chromatography has been developed and specifically adopted for the separation of serum lipoproteins into their respective alpha- and beta-lipoprotein fractions. The cholesterol, phospholipids, apoproteins, and triglycerides of these two lipoprotein fractions--the high-density lipoproteins (HDL) and lower density lipoproteins--can be directly measured independently after this separation. The sums of each fraction agree with total serum components when independently assayed. The affinity column is packed with heparin bound in high capacity to agarose. The beta-lipoproteins (low-density and very-low-density lipoproteins) and HDL-containing apolipoprotein E are absorbed to the column support; the alpha-lipoproteins (non-apolipoprotein E) pass through. The beta-lipoproteins are subsequently desorbed from the column support with saline. The direct assay of both the "protective" alpha-lipoprotein cholesterol (HDL cholesterol) and of the presumably atherogenic lower-density beta-lipoprotein cholesterol (LDL + VLDL cholesterol) permits calculation of a beta-: alpha-lipoprotein cholesterol ratio, which may better indicate a patient's risk of stroke or coronary heart disease than does the value for HDL cholesterol alone.
基于亲和色谱原理开发了一种快速实用的方法，并专门用于将血清脂蛋白分离成各自的α-脂蛋白和β-脂蛋白组分。在这种分离之后，可以直接独立测量这两种脂蛋白组分（高密度脂蛋白（HDL）和低密度脂蛋白）的胆固醇、磷脂、载脂蛋白和甘油三酯。当独立测定时，每个组分的总和与血清总成分一致。亲和柱填充有与琼脂糖高容量结合的肝素。β-脂蛋白（低密度和极低密度脂蛋白）以及含载脂蛋白E的HDL被吸附到柱载体上；α-脂蛋白（非载脂蛋白E）则通过。随后用盐水将β-脂蛋白从柱载体上解吸下来。对“保护性”α-脂蛋白胆固醇（HDL胆固醇）和可能具有致动脉粥样硬化作用的低密度β-脂蛋白胆固醇（LDL + VLDL胆固醇）进行直接测定，可以计算β-：α-脂蛋白胆固醇比值，与单独的HDL胆固醇值相比，该比值可能更能表明患者患中风或冠心病的风险。
